Center for Digital Agriculture

Mentors and projects

2023

Portrait of Angela Green-Miller

Dr. Angela Green-Miller

Associate Professor, Agricultural & Biological Engineering, UIUC College of ACES | Biography

Projects

Activity Index for Pigs Using Image Analysis

Abstract written by 2023 REU student Jelena Herriott.

 

Abstract

Ractopamine hydrochloride is a dietary supplement that increases growth rate when added to feed. The downside to using this supplement is an increase in agonistic behavior in pigs, and now an alternative supplement needs to be identified. The goal for this project is to develop an activity index model that can distinguish between various pig activity levels. This activity index will be developed by training a model that identifies low, medium, and high pig activity from image frames extracted from video. This Pig Activity Index Model (PAIM) is being developed to aid in solving the problem of efficiency of workers on farms, pig health, and the monitoring pig outcomes, such as during trials for new feed supplements. Pig activity can be used to determine the health status of pigs based on the change of activity levels, and the outcomes of this project will be used in future health detection tools.

 

This version of a PAIM utilized a learning model to recognize image differences to represent overall activity level. The development of the PAIM model used over 300 frames of groups of pigs representing a variety of behaviors and postures in six different pens of the same pig barn. Frames were categorized based on low, medium, and high activity level. Low pig activity is defined as lying and/or sitting. Medium pig activity is defined as maintenance behavior including: urinating, defecating, or feeding. High activity level is defined as exploring the pen, socializing, or agonistic behavior

Correlation of Image Analysis Metrics and Behavior of Pigs

Abstract written by 2023 REU student Scotteria Scott.

Alternative Title: Using Computer Vision Tools to Track Animal Movements

 

Abstract

Many studies have shown that using Computer Vision (CV) tools can help strength animal research through deep learning. CV plays a significant role in food production, animal welfare and their behavior.  The current goal of the study is to analyze an animal’s location and correlate it with their behavior to be able to predict an animal’s movement and activity. Within this project a Standard Operating Procedure (SOP) was created to document the steps of animal tracking and detection. The SOP breaks down the following detection models: YOLOV4, Label Studio, Detectron2, and Deep Simple Online Realtime Tracking (SORT). The detection models created an algorithm using the bounding boxes to create x and y coordinates tracking the animal. Using this method is important because it can contribute to detecting problems in animals before they happen and help animal scientist create solutions and solve them proactively. Applying their location and pairing it with their behavior can provide opportunities to further computer vision work in animal management. In the end of this study progress would have been made to correlate an animal’s location with their behavior and make farm life much easier.

Relevant Behavior and Posture for Emerging Illness in Pigs

Abstract written by 2023 REU student Emma Fuentes.

Alternative Title: Relevant Behavior and Posture for Emerging Respiratory Infection in Pigs

 

Abstract

Behavioral analysis could be utilized for the early detection of respiratory illness in sows. When executed with labor reducing computer technology, this could enable early treatment which could mitigate the economic and animal welfare costs of illnesses such as porcine reproductive and respiratory syndrome virus (PRRS), which burdens American breeding herds by an estimated $117.71 per breeding female and $302.6 million annually in additional costs and lost revenue (Holtkamp et al., 2012). The Behavioral Observation Research Interactive Software (BORIS (Friard & Gamba, 2016) was used to analyze behavioral footage of 10 sows subjected to a Lipopolysaccharide (LPS) immune challenge, which resulted in 3 mortalities. Postmortem evaluation revealed evidence of pre-existing subclinical respiratory conditions. A behavior ethogram, which consisted of 26 behavior labels, with paired postures, behaviors, and animal identifications was developed and applied between 10:30a.m. and 11:00a.m. shortly after the 2nd injections. This selected time frame served as a preliminary sample for anticipated further analysis between 10:30a.m. and 11:30a.m on the day prior to the trial and on the days of the 1st and 2nd injections. Behavior observations were continuously recorded for the selected time frame.

 

The goal of the behavior analysis of this study was to detect pigs with subclinical respiratory infections. Information about an animal’s health status gleaned from behavioral analysis could lead to achieving a more generous timeline for management intervention. Additionally, the methods of this experiment are supportive of an optimistic future for eventually training computer vision models for animal behavior analysis, which would relieve the pork industry of strain from labor shortages.

Portrait of Cabral Bigman-Galimore

Dr. Cabral Bigman-Galimore

Associate Professor, Communication, UIUC College of LAS | Biography

Projects

Exploring Bioinformatics Research Applications, Career Trajectories, and Social Implication

Abstract written by 2023 REU student Iradatulah Sulayman.

 

Abstract

This study addresses the need to understand the career paths, research projects, social-ethical implications of research in Bioinformatics and its application towards society. Our goal was to explore how students, graduates and professionals in bioinformatics reached their current positions and examine their perspectives on the social and ethical aspects of their work. We wanted to understand if society and experts in other fields have enough information about their research and how this engagement or lack thereof might perpetuate a narrative. The research methods comprise of in-depth interviews and literature reviews. In our interviews, qualitative methods of questioning were used and thematic analysis was applied to the data to identify recurring patterns and key themes. We used that to figure out what questions or areas need to be studied more and focused on aspects of bioinformatics that prior research finds controversial.

 

This study is important because it sheds light on the research and educational backgrounds of our interviewees while exploring their opinions on how their work affects society. It highlights their field’s significant contributions to digital biology and the agricultural system. It also dives into the ethical implications of their research, regarding food system, and the responsible use of technology. This study lays the groundwork for future research in the field of bioinformatics, regarding ethical considerations and will help continue to build and encourage responsible, impactful research practices. The findings contribute to the ongoing ethical discourse in Bioinformatics research and its impact on society.

Portrait of Isabella Condotta

Dr. Isabella Condotta

Assistant Professor, Animal Sciences, UIUC College of ACES | Biography

Projects

Computer Vision Systems Applied to Livestock Production

Abstract written by 2023 REU student Kennedie Manuel.

Alternative Title: Deep Learning Approach to Interpreting Tail Movement as an Estrus Sign in Gilts

 

Abstract

Timely estrus detection is vital to optimizing artificial insemination (AI) in gilts. Inaccurate insemination timing from missed estrus events is a major contributor to AI failure and can result in a loss of time, money, and reproductive output. Conventional methods rely heavily on human intervention, placing an undue burden on the depleting amount of farm labor. Some researchers have found that there may be a relationship between tail movement and estrus. This study aimed to create a computer vision-based object detection model using YOLO v8 (You Only Look Once) to detect estrus in gilts focusing on tail movements. Digital images of gilts were labeled for tail movement using Label Studio. The model was capable of detecting two tail positions (tail up, tail down) with an overall mean average precision (mAP) of 0.821 at 0.5 intersection over union (IoU). The mAP for tail up and tail down was 0.892 and 0.750, respectively. These results are satisfactory for detecting tail movement in gilts automatically. If appropriately implemented, this Artificial Intelligence model may lay the groundwork for expanding estrus detection technology to increase farm efficiency and farrowing rates.

Portrait of Lisa Ainsworth

Dr. Lisa Ainsworth

Professor, Integrative Biology, UIUC College of LAS | Biography

Projects

SoyFACE Data Compilation and Analysis

Abstract written by 2023 REU student Munirat Ibrahim.

Alternative Title: Visualizing and Analyzing the Effects of Ozone and Rainfall Exclusion on Soil Moisture Profiles

 

Abstract

Understanding the impacts of environmental factors on soil moisture profiles is very important for predicting how crops will respond to global climate change. In this study, we examined the effects of increased ozone concentration and rainfall exclusion on soil moisture dynamics. Soybean was grown under two ozone concentrations and two rainfall levels at the SoyFACE (Soybean Free Air Concentration Enrichment) facility. SoyFACE enables researchers to obtain essential insights into the possible effects of carbon dioxide and ozone pollution on crop productivity by using open-air field plots and controlled release of the gases to predict the impacts of future climate conditions on agriculture. In addition to ozone exposure, drought harms plants by reducing soil moisture levels and decreasing the amount of water available to plants. Soil moisture can be measured at various depths by inserting multiple probes into the soil, each equipped with sensors to measure soil moisture content at their respective depths. In order to understand the relationship between ozone exposure, drought, and soil moisture more clearly, we will visualize experimental soil moisture data using three-dimensional graphs. Three-dimensional graphs provide the advantage of improved visualization by representing numerous variables at once, allowing for a more thorough understanding of complex data. The intricate relationship between ozone concentration, rainfall, and soil moisture dynamics through time and space remains inadequately understood.

 

Our project aims to bridge this knowledge gap by utilizing agricultural data and mathematical coding techniques to analyze and visualize the effects of ozone and rainfall exclusion on soil moisture profiles.

Portrait of Matthew Hudson

Dr. Matthew Hudson

Professor, Crop Sciences, UIUC College of ACES | Biography

Projects

Exploring Bioinformatics Research Applications, Career Trajectories, and Social Implication

Abstract written by 2023 REU student Iradatulah Sulayman.

 

Abstract

This study addresses the need to understand the career paths, research projects, social-ethical implications of research in Bioinformatics and its application towards society. Our goal was to explore how students, graduates and professionals in bioinformatics reached their current positions and examine their perspectives on the social and ethical aspects of their work. We wanted to understand if society and experts in other fields have enough information about their research and how this engagement or lack thereof might perpetuate a narrative. The research methods comprise of in-depth interviews and literature reviews. In our interviews, qualitative methods of questioning were used and thematic analysis was applied to the data to identify recurring patterns and key themes. We used that to figure out what questions or areas need to be studied more and focused on aspects of bioinformatics that prior research finds controversial.

 

This study is important because it sheds light on the research and educational backgrounds of our interviewees while exploring their opinions on how their work affects society. It highlights their field’s significant contributions to digital biology and the agricultural system. It also dives into the ethical implications of their research, regarding food system, and the responsible use of technology. This study lays the groundwork for future research in the field of bioinformatics, regarding ethical considerations and will help continue to build and encourage responsible, impactful research practices. The findings contribute to the ongoing ethical discourse in Bioinformatics research and its impact on society.

Dr. Sarah Hind

Assistant Professor, Crop Sciences, UIUC College of ACES | Biography

Projects

Developing UV-Excitable GFP-Expressing Tomato Plants Using Hairy Root Transformation

Abstract written by 2023 REU student Valeria Suss.

 

Abstract
Tomatoes (
Solanum lycopersicum) rank as second for the most-consumed crop in the United States and are a model organism for studying fruit development, metabolic processes, and genetics. Plant transformation is a type of gene transfer that can naturally occur in the environment via infection by certain pathogenic bacteria, and has been utilized by scientists in the laboratory, allowing for significant advances in biotechnological research.  Generation of transgenic plants allows for faster implementation of traits of interest, thus leading towards development of more productive and resilient crops. Rhizobium rhizogenes, which is the causal agent of hairy root disease, causes the formation of transgenic or “hairy” roots on an infected plant.

 

The purpose of this study is to generate transformed tomato plants expressing an ultraviolet-excited Enhanced Green Fluorescent Protein (eYGFPuv) gene using hairy root transformation mediated by R. rhizogenes. Tomato seedlings were inoculated with a strain of R. rhizogenes (ATCC 15834) containing a plasmid encoding for eYGFPuv, and roots were monitored with a UV light for the expression of the reporter gene. We were able to induce hairy root transformation in tomato seedlings and observed eYGFPuv expression in all inoculated plants.

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